Thermo Fisher K8050-10 10 reactions TOPO XL-2whole PCRCloning kit, containing One Shot OmniMAX 2 T1<sup>R</sup>Chemically competent E. coli cells
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K8050-10 10 reactionsTOPO XL-2 Complete PCR Cloning Kit with One Shot OmniMAX 2 T1R Chemically Competent E. coli Cells
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TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of ultra-long PCR products (up to 13 kb) . This kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-ended PCR fragments. Platinum™ SuperFi™ Green PCR Master Mix is included in the kit to amplify long PCR fragments. Activation of topoisomerase I on the vector allows you to ligate PCR products on the test bench in just 5 minutes, achieving high cloning efficiency (up to 90% positive recombination with a 10 kb fragment).
pCR-XL-2-TOPO vector contains:
•The ccdB gene for positive selection
•The PCR product insertion site is flanked by EcoR I sites for easy excision of the inserted fragment
•Ampicillin and kanamycin resistance genes for selection in E. coli screening experiments
•T7 promoter/primer sites for in vitro RNA transcription and sequencing
•For sequencing T7, T3 and M13 Forward and Reverse Primer Sites
TOPO XL-2 Complete PCR Cloning Kit Includes:
•TOPO XL-2 PCR Cloning Kit Contains pCR-XL-2-TOPO Vectors
• Platinum SuperFi Green PCR Master Mix — Contains a proofreading DNA polymerase (guaranteed >300 times truer than Taq), with high processivity and density gradients to generate accurate, long PCR amplicons that can be loaded directly onto agarose gels for extraction
• PureLink™ Rapid Gel Extraction and PCR Purification Kit — Designed to purify DNA fragments from agarose gels , or perform direct PCR purification in less than 30 minutes using silica spin columns
• One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells — An improved chemically competent cell line ideal for a variety of cloning applications.
pCR-XL-2-TOPO vector contains:
•The ccdB gene for positive selection
•The PCR product insertion site is flanked by EcoR I sites for easy excision of the inserted fragment
•Ampicillin and kanamycin resistance genes for selection in E. coli screening experiments
•T7 promoter/primer sites for in vitro RNA transcription and sequencing
•For sequencing T7, T3 and M13 Forward and Reverse Primer Sites
TOPO XL-2 Complete PCR Cloning Kit Includes:
•TOPO XL-2 PCR Cloning Kit Contains pCR-XL-2-TOPO Vectors
• Platinum SuperFi Green PCR Master Mix — Contains a proofreading DNA polymerase (guaranteed >300 times truer than Taq), with high processivity and density gradients to generate accurate, long PCR amplicons that can be loaded directly onto agarose gels for extraction
• PureLink™ Rapid Gel Extraction and PCR Purification Kit — Designed to purify DNA fragments from agarose gels , or perform direct PCR purification in less than 30 minutes using silica spin columns
• One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells — An improved chemically competent cell line ideal for a variety of cloning applications.
For Research Use Only. Not for use in diagnostic procedures.
